Last data update: May 06, 2024. (Total: 46732 publications since 2009)
Records 1-2 (of 2 Records) |
Query Trace: Striley CAF[original query] |
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Assessment of exposure to PACs in asphalt workers: measurement of urinary PACs and their metabolites with an ELISA kit
Smith JP , Biagini RE , Johnson BC , Olsen LD , Mackenzie BA , Robertson SA , Sammons DL , Striley CAF , Walker CV , Snawder JE . Polycycl Aromat Compd 2011 31 (4) 270-285 An enzyme-linked immunosorbent assay (ELISA) kit made for determination of polycyclic aromatic compounds (PACs) in water was adapted for measuring PACs and their metabolites in urine. This method was then applied to a pilot asphalt worker PAC exposure study. Currently, liquid-liquid extraction with gas chromatography/isotope dilution high-resolution mass spectrometry (GC/HRMS) is the preferred method to determine urinary PAC metabolites. Although sensitive and specific, GC/HRMS is time consuming and costly. The ELISA method had a range from 14-720 ng/ml 1-hydroxypyrene equivalents with a lower limit of detection (LOD) of 14 ng/ml urine. ELISA and GC/HRMS PAC metabolite measurements had a statistically significant correlation and the PAC ELISA results were indicative of potential asphalt exposure. PAC ELISA is promising as a more rapid and less costly routine method for determining worker exposure to PACs in asphalt emissions. |
Use of direct reading surface sampling methods for site characterization and remediation of methamphetamine contaminated properties
Snawder JE , Striley CAF , Esswein EJ , Hessel J , Sammons DL , Robertson SA , Johnson BC , MacKenzie BA , Smith JP , Walker CV . J ASTM Int 2011 8 (6) JAI103481 Residual methamphetamine contamination in clandestine laboratories represents a hazard to emergency response personnel, remediation workers and the general public. To address this threat, two rapid, sensitive surface sampling techniques to assess the location and level of methamphetamine contamination were developed. Both methods employ established industrial hygiene surface sampling materials (wipes and swabs) but differ in their sensitivity and detection technology. One method, based on colorimetric disclosure, detects and confirms a collected sample or visible residues. The second method uses a lateral flow immunochemical assay (LFIA) for semi-quantitative detection of trace contamination. The National Institute for Occupational Safety and Health (NIOSH) partnered with public health agencies to develop applications of the methods for assessment of methamphetamine contamination of suspected properties. These applications focused on safe strategies for site assessment, hazard characterization, and remediation effectiveness. To conduct the field studies, NIOSH researchers and their partners visited more than a dozen suspected laboratories including mobile labs, abandoned properties, occupied residences, and motel rooms. NIOSH found greater than 95% agreement between positive identification of the presence of methamphetamine by LFIA and laboratory-based, liquid chromatography mass spectroscopy (LC- MS) methods. Test results were used to develop site assessments and make personal protective equipment recommendations. Results were also used to conduct process-based decontamination of properties and to make health-based decisions on remediation, re-occupancy of residences, as well as determine the degree of contamination of personal property in an inactive clandestine laboratory. By partnering with stakeholders, NIOSH was able to achieve two primary goals: (1) to develop a level of awareness in health department sanitarians, law enforcement personnel and other first responders that methamphetamine surface contamination was a potentially significant route of exposure; (2) to validate our methods in the field and to develop protocols for proper use and interpretation of the results. |
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